A new role for the plant growth-promoting nitrogen-fixing endophytic bacteria Gluconacetobacter diazotrophicus has been identified and characterized while it is. Gluconacetobacter diazotrophicus. This acid-tolerant organism is endophytic and colonizes internal plant tissues, establishing a symbiotic relationship with its. Gluconacetobacter diazotrophicus levansucrase is involved in tolerance to NaCl, sucrose and desiccation, and in biofilm formation.

Author: Feshura Tokazahn
Country: Senegal
Language: English (Spanish)
Genre: Automotive
Published (Last): 20 October 2005
Pages: 309
PDF File Size: 17.19 Mb
ePub File Size: 12.94 Mb
ISBN: 667-8-39709-709-1
Downloads: 82562
Price: Free* [*Free Regsitration Required]
Uploader: Mizshura

StrainInfo introduces electronic passports for microorganisms. These permits may be required for shipping this product: The bacterium is able to gain entry into its host plant through the roots, stems, or leaves [ 64 ]. Mutant Gluconacetobacter diazotrophicus PAL5 substrain B7 hypothetical protein gene, partial sequence.

Only first 5 entries are displayed. Treatments see Materials and Methods and Table 2. The aforementioned modes of entry for G. Moreover, we intend to initiate the molecular characterization of sugarcane- G.

Gluconacetobacter diazotrophicus Nitrogen-fixation

Results Biological activity characterization. T1 – one day; T2- three days; T3- seven days. Genome sequence analysis has shown that G. Mutant Gluconacetobacter diazotrophicus PAL5 substrain B1 putative glycosyl transferase gene, partial cds. An endophytic bacterium is being considered as an alternative or a supplemental way of reducing the use of chemicals in agriculture to control phytopathogens.

Production of indoleacetic acid and giberellins A 1 and A 3 by Gluconacetobacter diazotrophicus and Herbaspirillum seropedicae in chemically-defined culture media.

Bacterial detection by polymerase chain reaction. Prokaryotic Nomenclature Up-to-date – compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. This would be supported by the fact that X. Characterization of the plant genomic response. General characteristics of Gluconacetobacter diazotrophicus. As expected, in both blots control 18S rRNA transcript showed similar hybridization signals.

It harbors over species, some of which have high economic glucinacetobacter. International Journal of Systematic and Xiazotrophicus Microbiology.

Gluconacetobacter diazotrophicus (Gillis et al.) Yamada et al. ATCC &r

The Mo-nitrogenase is made up of two component proteins, the Fe protein containing the ATP-binding diasotrophicus and the MoFe protein containing the substrate binding sites [ 37 ]. However, oxygen is not the only inhibitor of nitrogenase, reactive oxygen species ROSby-products of aerobic metabolism critical in the production of ATP for the high energy-demanding process of nitrogen fixation, have also proven to be inhibitors of nitrogenase [ 3793 ].

Yamada Y, et al. Moreover, present results could be applied to other diazotrophicue from which recently Gluconacetobacter diazotrophicus has also been isolated, i. Native host plants of Gluconacetobacter diazotrophicus and the tissues from which they were discovered.

Sugarcane can specifically interact with Gluconacetobacter diazotrophicusa nitrogen-fixing bacterium. This fact could be explained by the lowest number of differentially expressed TDFs Mutant Gluconacetobacter diazotrophicus PAL5 substrain C9 putative xanthine dehydrogenase protein xdhC gene, partial cds.

In sugar cane Saccharum spp. Von Kruger et al. An additional method of inoculation specifically directed towards endophytic bacteria is foliar spraying [ 72 ]. With regard to the roots, G. With the sequenced gluconacetlbacter available to researchers, new strides have been made in understanding the many processes of G. Design was as it follows: Additionally, the nitrogenase of G. Principles, mechanisms of action, and future prospects.

Micropropagated sugarcane plants were both rooted and infested with G. Da Silva-Froufe and colleagues [ 26 ] have shown that using the same G. Cavalcante and Johanna Dobereiner. Confirmation of differential expression. While ROS levels were expected to increase during nitrogen fixation and elevated aerobic respiration, they in fact decreased within G.

International Journal of Agronomy

An additional mode of entry used by G. Both strains proceeded from the microorganism collection of the National Institute for Sugarcane Research, Havana, Cuba. Discussion The objective of this study was to use sugarcane plants, free of microorganisms, as a model to evaluate if G.

Future studies should put more emphasis on determining G. This paper will provide a comprehensive review and discuss the new progress on G. The objective of this study was to use sugarcane plants, free of microorganisms, as a model to evaluate if G. Expression of sugarcane genes induced by inoculation with Gluconacetobacter diazotrophicus and Herbaspirillum rubrisubalbicans.